Journal: Endocrinology

Article Title: Pulmonary GLP-1 receptor increases at birth and exogenous GLP-1 receptor agonists augmented surfactant-protein levels in litters from normal and nitrofen-treated pregnant rats.

doi: 10.1210/en.2012-1786

Figure Lengend Snippet: Figure 1. GLP-1R expression at consecutive stages of lung maturation in male and female rats. (Upper panel) Representative RT-PCR assay of GLP-1R gene expression at different stages of lung development. (Lower panel) Semiquantitative values (%OD) represent the mean SEM of 5 independent determinations (n 5): *P .05, P1 vs fetal period (E18 or E20); $P .05, Ad vs E18 or E20; #P .05, P1 vs Ad; &P .05, male vs corresponding female group (Kruskal-Wallis test followed by multiple comparisons). The amplification of 18S and GLP- 1R was detected after 10 and 25 amplification cycles, respectively. MWM, molecular weight marker: 100-bp DNA ladder; N, negative C with no added cDNA. Ad, adult.

Article Snippet: Primary antibody binding was detected using the following horseradish peroxidase (HRP)-conjugated secondary antibodies: antigoat IgG-HRP for SP-A (1:10 000, sc-2020; Santa Cruz Biotechnology, Inc) and antirabbit IgG-HRP for GLP-1R and -actin (1:5000, sc-2004; Santa Cruz Biotechnology, Inc).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Amplification, Molecular Weight, Marker

Journal: Endocrinology

Article Title: Pulmonary GLP-1 receptor increases at birth and exogenous GLP-1 receptor agonists augmented surfactant-protein levels in litters from normal and nitrofen-treated pregnant rats.

doi: 10.1210/en.2012-1786

Figure Lengend Snippet: Figure 5. GLP-1R expression in lungs from C and NTF-treated fetuses. (Upper panel) Representative RT-PCR analysis of GLP-1R expression. (Lower panel) Semiquantitative values (%OD) are represented as the mean SEM of 5 independent determinations (n 5): *P .05 (Kruskal-Wallis test followed by multiple comparisons). The amplification of 18S and GLP-1R was detected after 10 and 25 PCR cycles, respectively. MWM, molecular weight marker: 100-bp DNA ladder; N, negative C with no added cDNA.

Article Snippet: Primary antibody binding was detected using the following horseradish peroxidase (HRP)-conjugated secondary antibodies: antigoat IgG-HRP for SP-A (1:10 000, sc-2020; Santa Cruz Biotechnology, Inc) and antirabbit IgG-HRP for GLP-1R and -actin (1:5000, sc-2004; Santa Cruz Biotechnology, Inc).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

Journal: Journal of Nutritional Biology

Article Title: Insulin Receptor Levels Regulated by the Receptor- Associated Protein Progesterone Receptor Membrane Component 1 (PGRMC1)

doi: 10.18314/jnb.v4i2.1081

Figure Lengend Snippet: FIGURE 5 | Chronic effects of CLZ on hepatic PGRMC1-EGFR/GLP1R pathway and concentrations of PROG in plasma, liver and adrenal gland. (A) PGRMC1 (B) the ratio of p-EGFR/EGFR (C) GLP1R (D) PROG concentrations (ng/ml) in plasma (H = 12.53, p = 0.0138); (E) PROG concentrations (ng/g) in liver (H = 21.73, p = 0.0002); (F) PROG concentrations (ng/g) in adrenal gland (H = 20.15, p = 0.0005).

Article Snippet: Approximately 20 mg of protein was loaded, electrophoresed, blotted and then incubated with primary antibodies against PGRMC1, EGFR, GLP1R, PI3K p85, Akt, phospho-Akt (Ser473), GSK-3b, FOXO1, b-actin, PCNA (Proteintech Group, Wuhan, China), phospho-EGFR, phospho-GSK-3b (Affinity) overnight at 4°C.

Techniques: Clinical Proteomics

Journal: Journal of Nutritional Biology

Article Title: Insulin Receptor Levels Regulated by the Receptor- Associated Protein Progesterone Receptor Membrane Component 1 (PGRMC1)

doi: 10.18314/jnb.v4i2.1081

Figure Lengend Snippet: FIGURE 6 | Illustrative model of the mechanism focusing on hepatic PGRMC1 signaling underlying chronic CLZ-induced hepatic glucose disturbances. The add-on PGRMC1-OE can reverse CLZ-induced hepatic glucose disturbances by upregulating the expression of PGRMC1-EGFR/GLP1R-PI3K-Akt-GSK3b accompanied with the downregulation of nuclear FOXO1. CLZ, CLZ; AG205, the specific inhibitor of PGRMC1; PGRMC1-KD, the knockdown of PGRMC1; PGRMC1-OE, the overexpression of PGRMC1; H&E, hematoxylin and eosin staining; PAS, Periodic acid–Schiff staining; PGRMC1, PROG receptor membrane component 1; EGFR, epidermal growth factor receptor; GLP1R, glucagon-like peptide-1 receptor; PI3K, 3-phosphoinositide-dependent kinase-1; Akt, protein kinase B; GSK3b, glycogen synthase kinase-3b; FOXO1, the fork head box protein 1.

Article Snippet: Approximately 20 mg of protein was loaded, electrophoresed, blotted and then incubated with primary antibodies against PGRMC1, EGFR, GLP1R, PI3K p85, Akt, phospho-Akt (Ser473), GSK-3b, FOXO1, b-actin, PCNA (Proteintech Group, Wuhan, China), phospho-EGFR, phospho-GSK-3b (Affinity) overnight at 4°C.

Techniques: Expressing, Knockdown, Over Expression, Staining, Membrane

Journal: Gut Microbes

Article Title: A novel bile salt hydrolase-producing Ligilactobacillus salivarius prevents diet-induced obesity via regulation of bile acid metabolism and glucagon-like peptide 1 restoration

doi: 10.1080/19490976.2026.2668127

Figure Lengend Snippet: Alleviating effects of UDCA on hepatic functions through expressing BSH. Images of liver tissue (A) H&E and (B) Oil Red O staining. Scale bar, 100 μm. n = 4 mice per group. (C) The NAFLD activity score (NAS) of the liver tissue. (D) Oil Red O-stained area. The mRNA levels of (E) FXR, (F) SHP, (G) SREBP-1c, (H) PPARα, (I) AMPK, (J) GRP78, (K) CYP7A1, (L) TNF- α , (M) IL-6, and (N) IL-10. n = 4 mice per group. (O, P) Representative fluorescent images of SHP/FXR and AMPK/p-AMPK. (Q) IHC images of GLP-1R. Scale bar, 100 μm. n = 4 mice per group. (R–U) Positive area of FXR, SHP, AMPK/p-AMPK and GLP-1R. (V) The flowchart of the BSH inhibitor treatment experiment. (W) Concentration of UDCA in the ileum. n = 3 mice per group. (X) Concentration of GLP-1 in the serum. n = 4 mice per group. Student’s t-test to evaluate differences between two groups. * p < 0.05; ** p < 0.01; *** p < 0.001, # p < 0.05 (UDCA_low vs. UDCA_high).

Article Snippet: The sensor surface was activated by injecting a freshly prepared 1:1 mixture of 400 mM N -ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC) and 100 mM N -hydroxysuccinimide (NHS) at a flow rate of 10 μL/min for 420 s. Recombinant mouse GLP-1R protein (Catalog No. HY- P72206 , MedChemExpress) was diluted in 10 mM sodium acetate buffer (pH 4.5) to a concentration of 20 μg/mL and then immobilized onto the sample channel (Fc2) at 10 μL/min, achieving an immobilization level of approximately 12,600 response units (RU).

Techniques: Expressing, Staining, Activity Assay, Concentration Assay

Journal: Gut Microbes

Article Title: A novel bile salt hydrolase-producing Ligilactobacillus salivarius prevents diet-induced obesity via regulation of bile acid metabolism and glucagon-like peptide 1 restoration

doi: 10.1080/19490976.2026.2668127

Figure Lengend Snippet: UDCA reverses abnormalities in bile acid metabolism and dyslipidemia in glucose-treated HepG2 cells. (A) The viability of HepG2 cells treated with high glucose after different interventions. The mRNA levels of (B) FXR, (C) SHP, (D) SREBP-1c, (E) PPARα, (F) AMPK, (G) GRP78, and (H) CYP7A1. (I) Oil Red O-stained slices of HepG2 cells are shown. Scale bar, 50 μm. n = 4 mice per group. (J) Quantitative analyses of Oil Red O-stained slices of HepG2 cells are shown. (K) Representative fluorescent images of AMPK/p-AMPK. Scale bar, 100 μm. n = 4 mice per group. (L) Positive area of AMPK/p-AMPK. (M) Immunofluorescence staining of GLP-1R in HepG2 cells. Scale bar, 50 μm. n = 4 mice per group. (N) Positive area of GLP-1R. Molecular docking results of the (O) mouse and (P) human complexes over 200 ns MD simulations. Gibbs free energy of the (Q) mice and (R) humans. (S) Surface plasmon resonance (SPR) kinetic sensorgrams of UDCA binding to immobilized GLP-1R. (T) Steady-state affinity fitting curve. (U) Concentration of cAMP in HepG2 cells. n = 4 mice per group. Student’s t-test is used to evaluate differences between two groups. * p < 0.05; ** p < 0.01; *** p < 0.001, # p < 0.05 (UDCA_50. vs. UDCA_100; UDCA_200. vs. UDCA_100).

Article Snippet: The sensor surface was activated by injecting a freshly prepared 1:1 mixture of 400 mM N -ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC) and 100 mM N -hydroxysuccinimide (NHS) at a flow rate of 10 μL/min for 420 s. Recombinant mouse GLP-1R protein (Catalog No. HY- P72206 , MedChemExpress) was diluted in 10 mM sodium acetate buffer (pH 4.5) to a concentration of 20 μg/mL and then immobilized onto the sample channel (Fc2) at 10 μL/min, achieving an immobilization level of approximately 12,600 response units (RU).

Techniques: Staining, Immunofluorescence, SPR Assay, Binding Assay, Concentration Assay